Project
Part:BBa_K404127:Design
Designed by: Freiburg Bioware 2010 Group: iGEM10_Freiburg_Bioware (2010-10-11)
[AAV2]-left-ITR_pCMV_betaglobin_mVenus_[AAV2]-right-ITR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1319
Illegal AgeI site found at 2039 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Assembled vectorplasmid of BioBricks missing the hGH termiantion signal.
Source
Assembled vectorplasmid of BioBricks.
References
Danckwardt, S., Hentze, M.W. & Kulozik, A.E., 2008. 3' end mRNA processing: molecular mechanisms and implications for health and disease. The EMBO journal, 27(3), 482-98. Available at: http://www.ncbi.nlm.nih.gov/pubmed/18256699.
Millevoi, S. et al., 2006. An interaction between U2AF 65 and CF I(m) links the splicing and 3' end processing machineries. The EMBO journal, 25(20), 4854-64. Available at: http://www.ncbi.nlm.nih.gov/pubmed/17024186.